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Journal: Comprehensive Physiology
Article Title: Inhaled Halogen‐Induced Oxidative Renal Damage and Dysfunction: A Lung Heart Kidney Axis
doi: 10.1002/cph4.70096
Figure Lengend Snippet: Chlorine and bromine exposure leads to altered blood ion contents, albuminuria, and increased kidney injury biomarkers. Chlorine or bromine exposure was performed as described in the Methods section and above and animals were euthanized to collect arterial blood, urine, and kidney tissues. Sodium and potassium ion content were measured in the arterial blood by using a Heska EPOC machine (A and B), where white bars represent values in chlorine and gray bars represent values in bromine. Urine protein content was evaluated to assess renal damage (C), where white bars represent values in chlorine and gray bars represent values in bromine. Urine albumin (D), NGAL (E), KIM1 (F) and osteopontin (G) and RBP4 (H) were evaluated as part of a commercial kidney injury panel kit and an ELISA assay as described in the section. Western blotting was additionally performed to evaluate the injury marker NGAL in the renal tissues (I) where the top panel is for chlorine exposed tissue and the lower panel is for bromine exposed tissue and the densitometric evaluation of the bands with respect to β‐Actin loading control was also performed and shown in the lower panels (J) for chlorine and (K) for bromine. Values shown are Mean ± SEM ( n = 6–14). * Indicates p < 0.05 as compared to unexposed controls (naives).
Article Snippet:
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Marker, Control
Journal: Molecular & Cellular Proteomics : MCP
Article Title: Harmonized Peptide Libraries Enable Practical Biofluid Selection for Developing Biomarker Assays
doi: 10.1016/j.mcpro.2025.101086
Figure Lengend Snippet: Demonstration of the utility of CATalog for preliminary biofluid selection. ( A ) Retinol binding protein 4 as shown in CATalog ( left ) and in a secondary targeted experiment ( right ) in chronic kidney disease and healthy cats in plasma and serum (plasma p -value = 0.005, serum p -value = 0.613). ( B ) Leptin as shown in CATalog ( left ) and in a secondary targeted experiment ( right ) in chronic kidney disease and healthy cats in plasma and serum (plasma p -value = 0.027, serum p -value = 0.886).
Article Snippet: Here, we compare the relative abundance of
Techniques: Selection, Binding Assay, Clinical Proteomics